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A-375 人恶性黑色素瘤细胞
Cat NO.:HLX0014

General Information:

Organism:

Homo sapiens, human

Tissue:

skin

Culture Properties:

adherent

Morphology:

epithelial

Culture Method:

Complete Growth Medium:

DMEM (Gibco 12800-017)+10% FBS+ Penicillin/Streptomycin

Subculturing:

Volumes are given for a 625px2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Discard Trypsin-EDTA solution.
4. Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C, 5% CO2.

Subcultivation Ratio:

A subcultivation ratio of 1:3 to 1:6 is recommended

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase